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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2, I-Ad (H-2 Class II Histocompatibility Antigen, I-A beta Chain, H2-Eb1)

Anti -MHC Class 2, I-Ad (H-2 Class II Histocompatibility Antigen, I-A beta Chain, H2-Eb1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC IF
This antibody reacts with the I-Ad MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2d bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. This antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.
Catalog #M3887-11X
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.25ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG3,k
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
Immunogen(C3H x BALB/c)F1 mouse cells
SpecificityRecognizes mouse d and g7 haplotype. Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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