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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2, DQ alpha, Monomorphic (MHC Class II)

Anti -MHC Class 2, DQ alpha, Monomorphic (MHC Class II)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IP IH FC
The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain.
Catalog #M3887-40
ApplicationsSuitable for use in ELISA, Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunohistochemistry: Frozen sections
Immunohistochemistry (paraffin): Does not require protein digestion pre-treatment of paraffin sections. Does not require antigen retrieval using heat treatment prior to staining paraffin sections.
Western Blot: Reported as suitable
Optimal dilutions to be determined by the researcher.
HybridomaNSO myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F110
HostMouse
SourceSheep
ConcentrationNot Determined
FormSupplied as a liquid in 0.2M Tris-HCl, pH 7.4, 5-10% fetal calf serum, 0.01% sodium azide.
PuritySupernatant
ImmunogenAcid treated ovine MHC Class II
SpecificityRecognizes a monomorphic epitope on the alpha chain of sheep MHC class II DQ molecules which are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma. Species Crossreactivity: bovine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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