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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2, I-A (PE/CY5)

Anti -MHC Class 2, I-A (PE/CY5)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified FC
Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells.
Catalog #M3887-48D
Applications Flow cytometry
Complement-dependent cytotoxicity assays
Recommended DilutionFlow Cytometry: 0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No5K39
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE/CY5.
PurityPurified
SpecificityReacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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