| Catalog # | M3887-49E |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications have not been tested. |
| Recommended Dilution | Flow Cytometry: 1:50-1:100: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. |
| Immunohistochemistry (Frozen and paraffin sections): 1:50-1:100 |
| Note: This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)-refer to Whiteland et al. This clone is suitable for use on paraffin embedded material using target unmasking fluid-refer to McKechnie, N.M. et al., and has also been reported for application in Western Blot by Burrows, G.G. et al. (1998). This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.0M citrate buffer pH 6.0. |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo |
| I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo |
| Recommended Negative Controls | For rat tissues only |
| I1904-78Y: IgG1 Murine Negative Control. |
| Hybridoma | NS1 myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H440 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Rat Thymocyte membrane glycoprotein |
| Specificity | Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Crossreacts with certain mouse strains of MHC haplotypes k and s and analysis of recombinant strains showed that the determinants mapped to the I-A region of the mouse MHC. Species Crossreactivity: Mouse. Does not recognize BDIX rat strain. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
