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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2, I-B

Anti -MHC Class 2, I-B

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC IF
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections.
Catalog #M3887-49E
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended DilutionFlow Cytometry: 1:50-1:100: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry (Frozen and paraffin sections): 1:50-1:100. Requires antigen retrieval using heat treatment prior to staining for paraffin sections using sodium citrate buffer, pH 6.0. PLP fixation is recommended for optimal results. Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo
Recommended Negative Controls For rat tissues only
I1904-78Y: IgG1 Murine Negative Control.
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H440
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRat Thymocyte membrane glycoprotein.
SpecificityRecognizes a monomorphic determinant of the rat I-B antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Does not recognize BDIX rat strain. Crossreacts with certain mouse strains of MHC haplotypes k and s and analysis of recombinant strains showed that the determinants mapped to the I-A region of the mouse MHC. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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