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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2, I-A

Anti -MHC Class 2, I-A

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
Catalog #M3887-49F
IgG Goat anti mouse IgG RPE (rat adsorbed)
Fusion Partners Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Recommended Negative Controls Mouse IgG1 Negative Control
ApplicationsImmunohistochemistry: Frozen, Paraffin.
Western Blot
Flow Cytometry: 1:50-1:100
Optimal working dilutions to be determined by researcher.
Flow Cytometry Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H440
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRat Thymocyte membrane glycoprotein.
SpecificityRecognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells (2,3,4). The antibody cross-reacts with certain mouse strains of MHC haplotypes k and s and analysis of recombinant strains showed that the determinants mapped to the I-A region of the mouse MHC (1,2).* This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)-refer to Whiteland, J.L. (1995).This clone is suitable for use on paraffin embedded material using target unmasking fluid (HIS003)-refer to McKechnie, N.M. et al. (1997) and has also been reported for application in Western blotting by Burrows, G.G. et al. (1998). Species Crossreactivity: Mouse. Does not recognize BDIX rat strain (10).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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