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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH FC

Catalog #	M3887-49F
IgG Goat anti mouse IgG	RPE (rat adsorbed)
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Recommended Negative Controls	Mouse IgG1 Negative Control
Applications	Immunohistochemistry: Frozen, Paraffin.
	Western Blot
	Flow Cytometry: 1:50-1:100
	Optimal working dilutions to be determined by researcher.
Flow Cytometry	Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H440
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Rat Thymocyte membrane glycoprotein.
Specificity	Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells (2,3,4). The antibody cross-reacts with certain mouse strains of MHC haplotypes k and s and analysis of recombinant strains showed that the determinants mapped to the I-A region of the mouse MHC (1,2).* This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)-refer to Whiteland, J.L. (1995).This clone is suitable for use on paraffin embedded material using target unmasking fluid (HIS003)-refer to McKechnie, N.M. et al. (1997) and has also been reported for application in Western blotting by Burrows, G.G. et al. (1998). Species Crossreactivity: Mouse. Does not recognize BDIX rat strain (10).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.