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You are here:Home » Antibodies » Antibodies-MHC » Anti -MHC Class 2, I-A (FITC)

Anti -MHC Class 2, I-A (FITC)


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Clone Host Grade Applications
Monoclonal Mouse Purified FC
Catalog #M3887-49H
Fusion Partners Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Recommended Negative Controls Mouse IgG1 Negative Control: FITC
ApplicationsFlow Cytometry: Neat
Optimal working dilutions to be determined by researcher.
Flow Cytometry Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Clone TypeMonoclonal
Clone No3H435
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified IgG.
ImmunogenRat thymocyte membrane glycoproteins.
SpecificityRecognizes a polymorphic determinant of rat Ia antigen present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). The antibody crossreacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. It recognizes la antigens on B-cells, dendritic cells and certain epithelial cells (1, 3, 4). It is particularly useful for distinguishing la positive cells from different rat strains, eg for recognizing cells of donor origin in bone marrow reconstituted radiation chimeras (3,4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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