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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified FC

Catalog #	M3887-49J
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Recommended Negative Controls	Mouse IgG1 Negative Control: RPE
Applications	Flow Cytometry: Neat
	Optimal working dilutions to be determined by researcher.
Flow Cytometry	Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H440
Host	Mouse
Source	Rat
Concentration	~100 Tests/1ml
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 0.1% sodium azide, 1% BSA. Conjugated to R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Rat Thymocyte membrane glycoprotein.
Specificity	Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells (2,3,4). The antibody cross-reacts with certain mouse strains of MHC haplotypes k and s and analysis of recombinant strains showed that the determinants mapped to the I-A region of the mouse MHC (1,2). Species Crossreactivity: Mouse. Does not recognize BDIX rat strain (6).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.