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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified IH FC

Catalog #	M3887-49K
IgG Goat anti mouse IgG	RPE (rat adsorbed)
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Recommended Negative Controls	Mouse IgG1 Negative Control
Applications	Flow Cytometry: 1:100
	Immunohistochemistry: Frozen, Paraffin.
	Optimal working dilutions to be determined by researcher.
Flow Cytometry	Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H435
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Ion Exchange chromatography.
Immunogen	Rat thymocyte membrane glycoproteins.
Specificity	Recognizes a polymorphic determinant of rat Ia antigen (RT1.Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). The antibody crossreacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. It recognizes Ia antigens on B-cells, dendritic cells and certain epithelial cells (1,3,4). It is particularly useful for distinguishing Ia positive cells from different rat strains, eg for recognizing cells of donor origin in bone marrow reconstituted radiation chimeras (3,4.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.