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You are here:Home » Antibodies » Antibodies-MHC » Anti -MHC Class 2, I-A, Rt1.Bu

Anti -MHC Class 2, I-A, Rt1.Bu


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Clone Host Grade Applications
Monoclonal Mouse Highly Purified FC
Catalog #M3887-49L
Fusion Partners Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Recommended Negative Controls Mouse IgG1 Negative Control: RPE
ApplicationsFlow Cytometry: Neat
Optimal working dilutions to be determined by researcher.
Flow Cytometry Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Clone TypeMonoclonal
Clone No3H437
Concentration~100 Tests/1ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 0.1% sodium azide, 1% BSA. Conjugated to R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
PurityPurified by Ion Exchange chromatography.
ImmunogenRat thymocyte membrane glycoproteins.
SpecificityRecognizes a polymorphic determinant of rat Ia antigen (RT1.Bu) present on Lewis, Wistar and AO strain rats, but not RN, DA or PVG/c strains. The antibody crossreacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. It recognizes Ia antigens on B cell, dendritic cells and certain epithelial cells. It is particularly useful for distinguishing Ia positive cells from different rat strains, eg for recognizing cells of donor origin in bone marrow reconstituted radiation chimeras.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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