Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH FC IF

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.
Catalog #	M3887-49M
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 1:50-1:100 Use 10ul to label 10e6 cells in 100ul.
	Immunohistochemistry (Paraffin): 1:50-1:100. Antigen retrieval using heat treatment prior to staining of paraffin section is required. Sodium citrate buffer, pH 6.0 is recommended.
	Immunohistochemistry: Frozen sections
	Optimal dilution determined by the researcher.
Hybridoma	Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H440
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Rat thymocyte membrane glycoprotein
Specificity	Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Crossreacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region. Does not react with the rat BDIX strain due to a defect in RT1B expression. Species Crossreactivity: mouse
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.