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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class I (RT1A)

Anti -MHC Class I (RT1A)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Soluble MHC class I antigens can be detected in the serum of humans and various animals and appear in the circulation shortly after liver transplantation. The precise role of these antigens is currently uncertain, but soluble MHC class I may be involved in immunomodulation.
Catalog #M3887-49O
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50-1:100 Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen
Optimal dilution determined by the researcher.
HybridomaSpleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A444
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPHA activated rat lymphocytes
SpecificityRecognizes a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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