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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH FC

Soluble MHC class I antigens can be detected in the serum of humans and various animals and appear in the circulation shortly after liver transplantation. The precise role of these antigens is currently uncertain, but soluble MHC class I may be involved in immunomodulation.
Catalog #	M3887-49O
Applications	Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:50-1:100 Use 10ul to label 10e6 cells in 100ul.
	Immunohistochemistry: Frozen
	Optimal dilution determined by the researcher.
Hybridoma	Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	6A444
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	PHA activated rat lymphocytes
Specificity	Recognizes a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.