Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2, I-Ak/s (MHC Class II)

Anti -MHC Class 2, I-Ak/s (MHC Class II)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region. M3887-54B recognises a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. The literature reports that this clone does not react with the rat BDIX strain due to a defect in RT1B expression. M3887-54B also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region.
Catalog #M3887-54B
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (Paraffin): 1:50-1:100
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F125
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRat thymocyte membrane glycoproteins
SpecificityRecognizes rat MHC class II I-Ak/s. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links