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You are here:Home » Antibodies » Antibodies-MHC » Anti -MHC Class 2 (beta chain)

Anti -MHC Class 2 (beta chain)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
Human MHC Class II (HLA-DP, DQ & DR) molecules are expressed on dendritic cells, monocytes, macrophages, and myeloid cells. MHC Class II molecules are heterodimers of polymorphic transmembrane a and b chains and generally bind exogenously derived peptides of about 10-17 amino acids. MHC Class II molecules interact with the T cell receptor on CD4+ T cells.
Catalog #M3887-55A
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 5ug/ml
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. 5x10e5 cultured Daudi human tumor cells were incubated for 45 minutes on ice with 80ul of M3887-55A at 5ug/ml.
2. Cells were washed twice and incubated with 2º reagent Goat anti-Mouse IgG/FITC.
3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 2.16 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Recommended Secondary AntibodiesI1904-76L IgG1 (FITC) Pab Gt xMo
I1904-79L IgG1 (FITC) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No7H218
FormSupplied as a liquid in PBS, pH 7.5, 0.5mg/ml gentamicin sulfate.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified class II from JY cell line
SpecificityRecognizes a human monomorphic class II beta chain epitope.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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