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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2 (beta chain) (Biotin)

Anti -MHC Class 2 (beta chain) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
Human MHC Class II (HLA-DP, DQ & DR) molecules are expressed on dendritic cells, monocytes, macrophages, and myeloid cells. MHC Class II molecules are heterodimers of polymorphic transmembrane a and b chains and generally bind exogenously derived peptides of about 10-17 amino acids. MHC Class II molecules interact with the T cell receptor on CD4+ T cells.
Catalog #M3887-55B
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry: Frozen sections
Flow Cytometry: 1ug/ml labels 5x10e5 cultured human Daudi cells
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Five x 10e5 cultured human Daudi cells were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (To block non specific binding).
2. After which they were incubated for 45 minutes on ice with 80ul of M3887-55B at 1ug/ml.
3. Cells were washed twice and incubated with S7973-88P: Streptavidin (PE) after which they were washed three times, fixed and analyzed by FACS.
4. Cells stained positive with a mean shift of 2.40 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration.
5. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml M3887-55A: MHC Class 2 (beta chain).
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H218
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified class II from JY cell line
SpecificityRecognizes a human monomorphic class II beta chain epitope.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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