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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH FC

Human MHC Class II (HLA-DP, DQ & DR) molecules are expressed on dendritic cells, monocytes, macrophages, and myeloid cells. MHC Class II molecules are heterodimers of polymorphic transmembrane a and b chains and generally bind exogenously derived peptides of about 10-17 amino acids. MHC Class II molecules interact with the T cell receptor on CD4+ T cells.
Catalog #	M3887-55B
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: Frozen sections
	Flow Cytometry: 1ug/ml labels 5x10e5 cultured human Daudi cells
	Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol	1. Five x 10e5 cultured human Daudi cells were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (To block non specific binding).
	2. After which they were incubated for 45 minutes on ice with 80ul of M3887-55B at 1ug/ml.
	3. Cells were washed twice and incubated with 2º reagent S7973-88P: Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS.
	4. Cells stained positive with a mean shift of 2.40 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration.
	5. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml M3887-55A: MHC Class 2 (beta chain).
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	7H218
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Purified class II from JY cell line
Specificity	Recognizes a human monomorphic class II beta chain epitope.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.