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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B FC

Human MHC Class II (HLA-DP, DQ & DR) molecules are expressed on dendritic cells, monocytes, macrophages, and myeloid cells. MHC Class II molecules are heterodimers of polymorphic transmembrane a and b chains and generally bind exogenously derived peptides of about 10-17aa MHC Class II molecules interact with the T cell receptor on CD4+ T cells.
Catalog #	M3887-55C
Applications	Suitable for use in Flow Cytometry and Western Blot.
	Other applications not tested.
Recommended Dilutions	Flow Cytometry: 1:50 (5ug/ml) labels 5x10e5 human Daudi cells
	Optimal dilutions to be determined by the researcher.
FACS Protocol	1. Five x10e5 cultured human Daudi cells were pre-incubated
	with 20ul of human IgG at 300ug/ml (to block non-specific binding), after which they were incubated on ice for 45 minutes with 80ul of M3887-55C at a 1:50 dilution factor (5ug/ml).
	2. Cells were washed three times, fixed and analyzed by FACS.
	3. Cells stained positive with a mean shift of 2.1 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration.
	4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml M3887-55A MHC Class 2 (beta chain).
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	7H218
Host	Mouse
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, 5% Glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with FITC.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Purified MHC class II from JY cell line.
Specificity	Recognizes human monomorphic class II beta chain epitope.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.