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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IP IH FC

Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen.2
Catalog #	M3887-62F
Applications	• Flow cytometry1
	• Complement-dependent cytotoxicity1
	• Immunoprecipitation3
	• In vitro induction of antigen-specific B-cell differentiation4
	• In vitro blocking of antigen presentation5
	• Immunohistochemistry (acetone-fixed, frozen tissue sections)6
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	14-4-4S
Host	Mouse
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Purity	Purified
Immunogen	C3H mouse skin graft and splenocytes
Specificity	Recognizes an epitope on mouse I-Ek MHC class II alloantigen. Reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. Has been reported to crossreact with the rat class II alloantigen RT1D.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.