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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2, I-Ek (Azide Free)

Anti -MHC Class 2, I-Ek (Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP IH FC
Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen.2
Catalog #M3887-62FX
Applications Flow cytometry1
Complement-dependent cytotoxicity1
Immunoprecipitation3
In vitro induction of antigen-specific B-cell differentiation4
In vitro blocking of antigen presentation5
Immunohistochemistry (acetone-fixed, frozen tissue sections)6
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No14-4-4S
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS. No preservative added.
PurityPurified
ImmunogenC3H mouse skin graft and splenocytes
SpecificityRecognizes an epitope on mouse I-Ek MHC class II alloantigen. Reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. Has been reported to crossreact with the rat class II alloantigen RT1D.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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