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You are here:Home » Antibodies » Antibodies-MHC » Anti -MHC Class 2, I-Ek (Biotin)

Anti -MHC Class 2, I-Ek (Biotin)


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Clone Host Grade Applications
Monoclonal Mouse Purified IP IH FC
Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen.2
Catalog #M3887-62H
Applications Flow cytometry1
Complement-dependent cytotoxicity1
In vitro induction of antigen-specific B-cell differentiation4
In vitro blocking of antigen presentation5
Immunohistochemistry (acetone-fixed, frozen tissue sections)6
Recommended DilutionFlow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No14-4-4S
FormSupplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
ImmunogenC3H mouse skin graft and splenocytes
SpecificityRecognizes an epitope on mouse I-Ek MHC class II alloantigen. Reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. Has been reported to crossreact with the rat class II alloantigen RT1D.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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