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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IH FC

Reacts with the I-Ek MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. This antibody cross-reacts with cells from mice of the H-2d, H-2p, and H-2r haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. This antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-Ek.
Catalog #	M3887-62K
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Flow Cytometry: 0.25ug/10e6 cells in 100ul volume
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Host	Mouse
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	C3H mouse skin graft and splenocytes
Specificity	Recognizes mouse I-Ek MHC class II alloantigen. Crossreactivity: H-2d,p,r haplotypes and rat MHC class II antigen RT1D.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.