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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2, RT1B (MHC Class II)

Anti -MHC Class 2, RT1B (MHC Class II)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. The literature reports that this clone does not react with the rat BDIX strain due to a defect in RT1B expression. Also crossreacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region.
Catalog #M3887-67C
ApplicationsSuitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50-1:100
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoOX-6
HostMouse
SourceRat
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRat thymocyte membrane glycoprotein.
SpecificityRecognizes a monomorphic determinant of the rat MHC Class II RT-1B. Species sequence homology: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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