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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Mi-2

Anti -Mi-2

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Mi-2 is a nuclear protein originally characterized as an autoantigen in the human connective tissue disease dermatomyositis. It is a member of the SNF2/RAD 54 family of helicases involved in chromatin remodeling associated with genome replication, expression and repair and chromosome segregation. Mi-2 was recently identified as a component of NURD, a nucleosome remodeling and histone deacetylase complex.
Catalog #M3888
ApplicationsSuitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detected Mi-2 in Hela nuclear extract.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen ControlH1838: HeLa Nuclear Extract. Use 20ug per lane for minigels.
HeLa nuclear extract was resolved by electrophoresis transferred to nitrocellulose and probed with anti-Mi-2 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, containing 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenFusion protein corresponding to amino acid residues 474970 of human Mi-2.
SpecificityRecognizes human Mi-2 at 218kD and additional proteins at 150, 80, 46, 36kD. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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