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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Mi-2 is a nuclear protein originally characterized as an autoantigen in the human connective tissue disease dermatomyositis. It is a member of the SNF2/RAD 54 family of helicases involved in chromatin remodeling associated with genome replication, expression and repair and chromosome segregation. Mi-2 was recently identified as a component of NURD, a nucleosome remodeling and histone deacetylase complex.
Catalog #	M3888
Applications	Suitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended Dilution	Western Blot: 0.5-2ug/ml detected Mi-2 in Hela nuclear extract.
	Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control	H1838: HeLa Nuclear Extract. Use 20ug per lane for minigels.
	HeLa nuclear extract was resolved by electrophoresis transferred to nitrocellulose and probed with anti-Mi-2 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, containing 30% glycerol.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Fusion protein corresponding to amino acid residues 474–970 of human Mi-2.
Specificity	Recognizes human Mi-2 at 218kD and additional proteins at 150, 80, 46, 36kD. Species Crossreactivity: Human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.