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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition (-D-Ala-L-X-D-erythro-b-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa -amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa. With a direct competitive enzyme-linked immunosorbent assay (ELISA) using this antibody, IC50 values for microcystin-LR of 0.06ug/l have been obtained. The provisional guideline value proposed by the WHO is 1ug/l for drinking water. The detection limit for microcystin-LR is 0.006ug/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for this product was determined to be at least 7 x 10e10 l/mol. The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1ug/l were measured and a mean recovery of 99.9 ± 16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.
Catalog #	M3889-25A
Hybridoma	Mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with Microcystin-LR-BSA conjugate.
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10J35
Host	Mouse
Concentration	As reported
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Microcystin-LR-BSA conjugate.
Specificity	Recognizes Microcystin-LR.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.