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Migration Inhibitory Factor, Recombinant, Human (Macrophage MIF, Glycosylation-Inhibiting Factor, GIF, GLIF, MMIF)
Migration Inhibitory Factor, Recombinant, Human (Macrophage MIF, Glycosylation-Inhibiting
Factor, GIF, GLIF, MMIF)
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| Macrophage migration inhibitory activity originally identified 30 years ago based on the ability to prevent the migration of guinea pig macrophages out of capillary tubes in vitro. A number of cytokines, including interferon-g and IL-4, exhibit macrophage migration inhibitory activity. A cDNA clone was isolated from a lectin-stimulated T-cell hybridoma cDNA library and reported to encode a protein with macrophage migration inhibitory activity. This protein was referred to as human migration inhibitory factor (MIF). MIF cDNA encodes a 115 amino acid residue polypeptide that lacks conventional hydrophobic leader sequence. MIF is expressed in many tissues and in all cell lines examined to date. MIF was also discovered as a growth factor-induced delayed early response gene DER6. Recently, MIF was reported to be a secreted cytokine that exerts a central and wide ranging role in host inflammatory responses. Evidence for a receptor of this has not been reported. E. coli-expressed MIF lacks macrophage migration inhibitory activity and is recommended for use as a calibrator for MIF immunoassays. | | | Catalog # | M3895-07 | | Purified recombinant human MIF contains 114 amino acid residues and lacks the N-terminal methionyl group. Recombinant MIF has a predicted molecular mass of approximately 12.5kD. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute with sterile PBS,, | | > 0.1% BSA or HSA. Aliquot and store at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~12.5kD | | Source | Human, recombinant protein expressed in E. coli. | | Purity | ~ 97% as determined by N-terminus analysis and SDS-PAGE (silver stain). | | Form | Supplied as a lyophilized powder from 100mM Tris, pH 8.0 and 50ug BSA/ug protein. Reconstitute with sterile PBS, > 0.1% BSA or HSA to 10ug/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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