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You are here:Home » Molecular Biology » Growth Factors-MIF » MIF, Recombinant, Human, Western Blot Control (Macrophage Migration Inhibitory Factor, Glycosylation-inhibiting Factor, GIF, L-dopachrome Isomerase, L-dopachrome Tautomerase, Phenylpyruvate Tautomerase, GLIF, MMI)

MIF, Recombinant, Human, Western Blot Control (Macrophage Migration Inhibitory Factor,
Glycosylation-inhibiting Factor, GIF, L-dopachrome Isomerase, L-dopachrome Tautomerase,
Phenylpyruvate Tautomerase, GLIF, MMI)

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Specifications

MIF is involved in cell-mediated immunity, immunoregulation, and inflammation. Reportedly, it also plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signalling pathways. However, the biological mechanism and significance of MIF remains unknown.
Catalog #M3895-07U
SourceRecombinant corresponding to aa101-445 from mature human MIF, fused to 6x His-tag at N-terminal, expressed in E. coli.
ApplicationsSuitable for use in Western Blot as a control. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
PurityPurified (SDS-PAGE)
ConcentrationAs reported
FormSupplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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