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You are here:Home » Molecular Biology » Growth Factors-Midkine » Midkine, Recombinant, Human (MK, Neurite Growth Promoting Factor-2, NEGF-2)

Midkine, Recombinant, Human (MK, Neurite Growth Promoting Factor-2, NEGF-2)

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Specifications

Human Midkine (MK) is a new member of the heparin-binding neurotrophic factor family. MK and PTN (Pleiotrophin) are structural homologs, and are highly conserved among species. MK plays important roles in development and carcinogensis and has several important biological effects, including promotion of neurite extension and neuronal survival. Recombinant Midkine is a 13.4kD protein, comprising of 123 amino acid residues.
Catalog #M3896
Biological ActivityDetermined by its ability to chemoattract human neutrophils using a concentration range of 0.1-10ng/ml.
AA Sequence
VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile dH2O, and a carrier protein. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
Molecular Weight13.4kD
SourceE. coli
Purity98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug).
FormSupplied as a sterile filtered, lyophilized powder from PBS, pH 7.4. Reconstitute with sterile dH2O, carrier protein to 0.1-1mg/ml
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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