Midkine, Recombinant, Human (MDK, MK, Neurite Growth Promoting Factor-2, NEGF-2)
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| Midkine, also known as Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor of low molecular weight, a member of the NEGF family whose founding member is pleiotrophin. This protein exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. Recombinant human Midkine protein, fused to His-tag at N-terminus, , expressed in E. coli and purified by using conventional chromatography. | | | Catalog # | M3896-03 | | Source | Recombinant human Midkine, aa21-143, His-tagged, expressed in E. coli | | AA Sequence | MGSSHHHHHH SSGLVPRGSH MVAKKKDKVK KGGPGSECAE WAWGPCTPSS KDCGVGFREG TCGAQTQRIR CRVPCNWKKE FGADCKYKFE NWGACDGGTG TKVRQGTLKK ARYNAQCQET IRVTKPCTPK TKAKAKAKKG KGKD | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 15.7kD | | Source | E.coli | | Purity | ~90% by SDS-PAGE Purified by immunoaffinity chromatography. | | Form | Supplied as liquid in 20mM Tris-HCl buffer, pH8.0, 0.1 M NaCl 2mM DTT, and 20% Glycerol | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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