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You are here:Home » Molecular Biology » Growth Factors-Midkine » Midkine, Recombinant, Human (MK, Neurite Growth Promoting Factor-2, NEGF-2)

Midkine, Recombinant, Human (MK, Neurite Growth Promoting Factor-2, NEGF-2)

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Specifications

Midkine (MK) is the product of a retinoic acid responsive gene, MK, and is a member of a family of heparin binding factors. It contains 121 amino acid residues including 10 conserved cysteine residues, all of which appear to be disulfide linked. Midkine is expressed during embryogenesis, showing an expression pattern that suggests functions in neurogenesis, cell migration, secondary organogenetic induction, and mesoderm-epithelial interaction. The widespread downregulation of MK in the adult human is reverted in a number of cancers, in which polypeptides are able to act as both transforming growth factors and promoters of angiogenesis. Midkine (MK), induces chemotaxis of human neutrophils and was found to trigger mobilization of intracellular calcium of these cells. Midkine induces histamine release from rat peritoneal mast cells with a rapid response in a dosedependent manner. Midkine is also a potent stimulator of collagen and glycosaminoglycan synthesis.
Catalog #M3896B
Recombinant Human Midkine is manufactured with N-terminal fusion of HisTag, having a molecular mass of 14.6kD protein and containing 121 amino acid residues of the human Midkine and 10 additional amino acid residues-HisTag
SequenceKKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D
SpecificityThe amino acid sequence of the recombinant human Midkine is 100% homologous to the amino acid sequence of the human Midkine without signal sequence.
ApplicationsSuitable for use in Western blot and ELISA.
Other applications have not been tested.
FormSupplied as a lyophilized powder from PBS, pH 7.2.
Reconstitute with 200ul PBS.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight14.6kD
SourceE. coli
Purity95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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