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You are here:Home » Growth Factors, Cytokines » Growth Factors-MIG » MIG, Recombinant, Mouse (Monokine induced by Interferon Gamma)

MIG, Recombinant, Mouse (Monokine induced by Interferon Gamma)

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Specifications

CXCL9 is a chemoattractant for activated T cells and possess angiostatic activity. Both CXCL9 and CXCL10 have been considered as important components for the anti-tumor activities of interferon-gamma (IFNgamma) and interleukin-12 in animal models. Mouse MIG is induced in THP-1 cells and in peripheral blood mononuclear cells by interferon-gamma but not by interferon-alpha or by lipopolysaccharide. Analysis of mouse and human genomic DNAs suggested that the Mumig and Humig genes are true mouse-human homologues.
Catalog #M3949-05A
DescriptionRecombinant Mouse MIG (monokine induced by gamma-interferon ) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 105 amino acids and having a molecular mass of 12208 Dalton. Recombinant Mouse MIG is purified by proprietary chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be, Thr-Leu-Val-Ile-Arg.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityMIG is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human lymphocytes using a concentration of 0.1-1 ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of Murine MIG.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a calibrated solution of Murine MIG as a Reference Standard.
SolubilityIt is recommended to reconstitute the lyophilized MIG in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized MIG although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MIG should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight12.21kD
SourceE. coli
Purity98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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