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You are here:Home » Antibodies » Antibodies-Interferon » Anti -MIG (Monokine induced by Interferon Gamma)

Anti -MIG (Monokine induced by Interferon Gamma)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Catalog #M3949-10A
ApplicationsSuitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.
Recommended DilutionNeutralization: To yield one-half maximal inhibition [ND50] of the biological activity (100ng/ml), a concentration of 5-7ug/ml is required.
ELISA Using 100ul/well antibody solution, requires a concentration of at least 0.5ug/ml. This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well.
Western Blot 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypePolyclonal
FormSupplied as a lyophilized powder from 0.5X PBS, pH 7.4. When reconstituted in sterile water to a concentration of >0.2mg/ml the antibody is stable for at least six weeks at 2-4°C.
PurityPurified by affinity chromatography.
ImmunogenHighly purified (>98%) recombinant hMIG (human MIG).
SpecificityRecognizes MIG
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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