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Specifications

Catalog #	M3975
Appearance	Light bluish beige, homogeneous, free flowing powder
Solubility	Deep royal blue, trace to slightly hazy
pH	7.0±0.4
Microbiological Tests	Cultures were incubated at 35ºC and examined for growth at 18-48 hours
Escherichia coli ATCC 25922	Partial to complete inhibition
	Staphylococcus aureus ATCC 25923: Partial to complete inhibition
	Streptococcus mitis ATCC 9811: Growth; blue colonies
	Streptococcus pyogenes ATCC 19615: Growth; blue colonies
	Streptococcus salivarius ATCC 13419: Growth; blue “gum drop” colonies
Formulation (g/L)	Casein digest of Peptone: 15g
	Peptic digest of Animal Tissue: 5g
	Sucrose: 50g
	Dextrose: 1g
	Dipotassium Phosphate: 4g
	Trypan Blue: 0.075g
	Crystal Violet: 0.0008g
	Agar: 15g
Directions per Liter	Dissolve 90 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Cool to 45-50ºC and aseptically add 1ml of a 1% potassium tellurite solution.
Storage and Stability	Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.