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You are here:Home » Antibodies » Abs to Mitochondrial Proteins » Anti -Mitochondria

Anti -Mitochondria

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Murine anti-mitochondria monoclonal antibody produces a "spaghetti"-like staining pattern in the cytoplasm of normal and malignant human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. This monoclonal antibody is part of a new panel of reagents which recognize subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues and biochemical preparations.
Catalog #M3985
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry:(formalin/paraffin) 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cells, Liver
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.424
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also supplied without BSA and sodium azide. See M3985X.
PurityPurified by Protein G affinity chromatography.
ImmunogenSemi-purified mitochondrial preparation. Cellular Localization:
Cytoplasmic.
SpecificityRecognizes a human 60kD non-glycosylated protein component of mitochondria found in human cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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