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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

This monoclonal antibody is part of a new panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb-2 produces a spaghetti-like staining pattern in the cytoplasm of normal and malignant human cells and may be used to stain the mitochondria in paraformaldehyde fixed frozen and formalin-fixed, paraffin-embedded tissues.
Catalog #	M3985-07
Cellular Localization	Immunohistochemically, this antibody produces a spaghetti like staining pattern in the cytoplasm of normal and malignant human cells.
Positive Control	HeLa cells. Liver tissue sections.
Applications	Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry FFPE/Fr. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50%glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8L782
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	10mM PBS [pH7.4] with 0.2%BSA
Purity	Purified by Protein G affinity chromatography.
Immunogen	Semi purified mitochondrial preparation. Epitope: This antibody recognizes a 60kD non glycosylated protein component of mitochondria found in human cells.
Specificity	Recognizes a 60kD non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. Species Crossreactivity: Crossreacts with Human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.