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You are here:Home » Antibodies » Abs to Mitochondrial Proteins » Anti -Mitochondria (BSA & Azide Free)

Anti -Mitochondria (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
This monoclonal antibody is part of a new panel of reagents which recognize subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues and biochemical preparations. Murine anti-mitochondria monoclonal antibody produces a "spaghetti"-like staining pattern in the cytoplasm of normal and malignant human cells
Catalog #M3985X
ApplicationsSuitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 minutes at RT.
Note: Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cells and liver
Cellular LocalizationCytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.424
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservative added. No stabilizing protein added. Also supplied with BSA and sodium azide. Refer to M3985.
PurityPurified by Protein G affinity chromatography.
ImmunogenSemi-purified mitochondrial preparation.
SpecificityRecognizes a human 60kD non-glycosylated protein component of mitochondria found in human cells. Epitope
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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