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You are here:Home » Antibodies » Antibodies-Golgi Proteins » Anti -Membrin (GS27, Golgi SNARE of 27K)

Anti -Membrin (GS27, Golgi SNARE of 27K)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Membrin, also known as GS27 (Golgi SNARE of 27K), is a 27kD integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport (1). GS27 is a member of a protein complex that consists of cis-Golgi vesicle receptor syntaxin 5 and GS28, v-SNARE rbet1 and rsec22, and rsly1. Immunoprecipitation analysis has shown that there are two subcomplexes within this complex: one contains syntaxin 5 and GOS28 and the other one contains syntaxin 5, GS27/membrin, rsec22 and rbet1 (2,3). These complexes are postulated to direct the fusion of ER-derived vesicles with Vesicular tubular cluster (VTC), and the fusion of VTCs to form cis-Golgi compartment (2,3).
Suitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested.
Catalog #M4149-10
Recommended DilutionWestern Blot (ECL): 2ug/mL
Immunoprecipitation: 2-4ug/mL
Immunocytochemistry: 1:500 (4)
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4E157
FormSupplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant rat membrin with a His6 tag
SpecificityThis affinity purified antibody detects an ~27kD protein, corresponding to the apparent molecular mass of membrin, on SDS-PAGE immunoblots in samples from human, monkey, rat, canine, chicken, hamster, porcine and rabbit origins
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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