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You are here:Home » Antibodies » Abs to Monocarboxylate Transporter » Anti -Monocarboxylate Transporter 1 (MCT1, MOT1)

Anti -Monocarboxylate Transporter 1 (MCT1, MOT1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF
Monocarboxylates such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid, followed by an increase in pH, leading to inhibition of glycolysis. Some tissues, such as brain, heart and red skeletal muscle, readily oxidize lactic acid. These tissues must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.
Catalog #M4470-02
ApplicationsSuitable for use in ELISA, Western Blot., Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionsWestern Blot: 1-5ug/ml using Chemiluminescence.
ELISA: 1:10,000-1:100,000. Control peptide (Cat #M4470-02A) can be used to coat ELISA plates at 50-100ng per well.
Immunohistochemistry: 3-10ug/ml in neutral buffered formaldehyde fixed tissue
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 17aa sequence within the cytoplasmic C-terminus of rat MCT1, conjugated to KLH. Species Sequence Homology
SpecificityRecognizes rat Monocarboxylate Transporter 1 (MCT1). No significant sequence homology with other MCTs.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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