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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » Mph1103I (AvaIII)

Mph1103I (AvaIII)

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Specifications

5'-A T G C A^T-3'
3'-T^A C G T A-5'
Catalog #M4680
SourceMoraxella phenylpyruvica RFL1103
Concentration 10u/ul
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
R1625-04 (Included)Restriction Enzyme Buffer E, 10X Incubate at 37°C
R1625 (Included)Restriction Enzyme Buffer A, 10X
Provided to for double digests.
Diluent Buffer10mM Tris-HCl, pH 7.4 at 25°C, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
Storage Buffer10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA,50% glycerol.
Enzyme PropertiesStability during Prolonged Incubation: A minimum of 0.3units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Methylation EffectsDam/Dcm/CpG/EcoKl: never overlaps- no effect
EcoBl: may overlap-effect not determined
Thermal InactivationEnzyme is inactivated by incubation at 65°C for 20min.
Compatible Ends Alw21I, PstI, SdaI, SduI
Number of Recognition Sites in DNALambda: 14
PhiX174: 0
M13mp18/19: 0
pBR322: 0
pUC18/19: 0
pUC57: 1
pTZ19R/U: 0
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffe
Quality Control
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Mph1103I.
Ligation/Recutting AssayAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with Mph1103I, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with10units of restriction endonuclease for 4 hours.
Blue/White Cloning AssayPUC57 was digested at a unique site with 10units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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