Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » MB-Enzymes, Restriction » Mph1103I (AvaIII)

Mph1103I (AvaIII)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Catalog #M4680
Sequence5'-A T G C A^T-3'
3'-T^A C G T A-5'
SourceMoraxella phenylpyruvica RFL1103
Concentration 10u/ul
Unit DefinitionOne unit is defined as the amount of Mph1103I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
Supplied withR1625: Restriction Enzyme Buffer A, 10X:
Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
R1625-04 Restriction Enzyme Buffer, 10X:
Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM potassium chloride,, 0.1mg/ml BSA
Storage BufferSupplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Enzyme PropertiesStability during Prolonged Incubation: A minimum of 0.3 units of Mph1103I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Methylation EffectsDam/Dcm/CpG/EcoKl: never overlaps- no effect
EcoBl: may overlap-effect not determined
Thermal InactivationMph1103I is inactivated by incubation at 65°C for 20min.
Compatible Ends
Alw21I, PstI, SdaI, SduI
Number of Recognition Sites in DNALambda: 14
PhiX174: 0
M13mp18/19: 0
pBR322: 0
pUC18/19: 0
pUC57: 1
pTZ19R/U: 0
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quality Control
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Mph1103I.
Ligation/Recutting AssayThe ligation and recleavage assay was replaced with LO test after validating experiments showed LO test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Mph1103I for 4 hours.
Blue/White Cloning AssayThe B/W assay was replaced with LO test after validating experiments showed LO test ability to detect nuclease and phosphatase activites with sensitivity that equals to that of B/W test.


External Links