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M4692-68F Rabbit Anti-Mst3, CT (Mammalian STE20-like Protein Kinase 3, MST-3, MST3B, Serine/threonine Kinase 24 (Ste20, Yeast Homolog), STE20 Homolog (yeast), STE20-like Kinase MST3, STK3, STK24)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q9Y6E0
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
EC=2.7.11.1, Serine/threonine-protein Kinase 24

The yeast 'Sterile 20' gene (STE20) functions upstream of the mitogen-activated protein kinase (MAPK) cascade. In mammals, protein kinases related to STE20 can be divided into 2 subfamilies based on their structure and regulation. Members of the PAK subfamily (see PAK3; MIM 300142) contain a C-terminal catalytic domain and an N-terminal regulatory domain that has a CDC42 (MIM 116952)-binding domain. In contrast, members of the GCK subfamily (see MAP4K2; MIM 603166), also called the Sps1 subfamily, have an N-terminal catalytic domain and a C-terminal regulatory domain without a CDC42-binding domain. STK24 belongs to the GCK subfamily of STE20-like kinases (Zhou et al., 2000 [PubMed 10644707]).[supplied by OMIM]

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human MST3 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human MST3.
References
1.Huang, C.Y., et al., J. Biol. Chem. 277(37):34367-34374 (2002). 2.Christian, S.L., et al., Genomics 79(5):635-656 (2002). 3.Zhou, T.H., et al., J. Biol. Chem. 275(4):2513-2519 (2000). 4.Schinkmann, K., et al., J. Biol. Chem. 272(45):28695-28703 (1997).
USBio References
No references available
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