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You are here:Home » Culture Media » Media-Microbiological » m-TEC Agar (Powder)

m-TEC Agar (Powder)

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Specifications

m-TEC Agar is used with urea for the isolation and enumeration of thermotolerant E. coli from water using the membrane filtration technique. E. coli is used as an indicator of fecal pollution in water. Researchers have been able to to quantitate E. coli on m-TEC Agar with 24 hours without requiring subculture and identification of isolates. m-TEC is an acronymn for membrane thermotolerant E. coli.
Catalog #M4693
Enzymatic digest of animal tissue provides nitrogen, carbon and minerals in m-TEC Agar. Yeast extract is a source of vitamins and trace elements. Lactose serves as a carbon source. Potassium Phosphate is the buffering agent. Sodium Lauryl Sulfate and Sodium Deoxycholate are selective agents against gram-positive bacteria. Bromcresol Purple and Bromphenol Red are pH indicators. Sodium Chloride maintains the osmotic balance of the medium. Agar is the solidifying agent.
Precaution
IRRITANT. Irritating to eyes, respiratory system and skin.
ApparanceSupplied as light grey-green-beige homogeneous, free-flowing powder.
SolubilityDark purple, clear to trace hazy.
Expected Cultural Responses.
Response on m-TEC Agar at 44.5ºC after 24-48 hours incubation
Response Reaction w/ Urease Substrate
Enterococcus faecalis (ATCC 29212) inhibited -
E. coli (ATCC 8738) growth yellow colonies
E. coli (ATCC 35150) growth yellow colonies
E. Coli (ATCC 35218) growth yellow colonies
Pseudomonas aeruginosa inhibited -
(ATCC 27853)
Results.
Yellow to yellow-brown colonies (urease-negative) may be presumptively identified as E. coli.
Storage and Stability.
Store sealed bottle containing the dehydrated medium at 4ºC. Once opened and recapped, place container in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping container tightly closed.


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