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You are here:Home » Antibodies » Abs to Mucin » Anti -Mucin 1 (MUC1 Glycoprotein, MUC-1, Breast Carcinoma Associated Antigen DF3, CA 15-3, Carcinoma Associated Mucin, CD227, DF3, EMA, Episialin, Epithelial Basement Membrane Antigen, Epithelial Membrane Antigen, Epithelial Mucin Tandem Repeat Sequence, H23 An

Anti -Mucin 1 (MUC1 Glycoprotein, MUC-1, Breast Carcinoma Associated Antigen DF3, CA 15-3,
Carcinoma Associated Mucin, CD227, DF3, EMA, Episialin, Epithelial Basement Membrane
Antigen, Epithelial Membrane Antigen, Epithelial Mucin Tandem Repeat Sequence, H23 An

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. 6378-0150 recognises CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.
Catalog #M4700-06H
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. This antibody has been tested on frozen human ovarian carcinoma tissue sections and paraffin embedded sections from normal breast tissue. A weak reaction is observed with sections of small intestine and colon.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10B1087
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman breast cancer cell line ZR-75-1.
SpecificityRecognizes human CD227. Epitope: 7-mer TSAPDTR of the CD227 tandem repeat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCD227 Antigen


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