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You are here:Home » Antibodies » Abs to Mucin » Anti -Mucin 1 (MUC1 Glycoprotein, CD227)

Anti -Mucin 1 (MUC1 Glycoprotein, CD227)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Mucins are heavily glycosylated glycoproteins, which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6,7 and 8) have been identified. Various immunohistochemical and in situ hybridization studies have shown that these mucins are differentially expressed in epithelia with cell-type specificity. Muc-5AC and Muc-6 glycoproteins are the first two mucins found in superficial epithelium and Muc-6 glycoprotein is found in the deep glands.
Catalog #M4700-07
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsELISA: Suitable for the detection of under-glycosylated tumor-associated MUC1 mucins in human serum.
Immunohistochemistry (FFPE and Frozen): 50ug/ml or less
Optimal dilutions to be determined by the researcher.
Affinity Constant>1x10e8
HybridomaX63-Ag8-653 myeloma cells with spleen cells from BALB/c mice
Recommended ELISA pairsCapture: M4700-08 or M4700-05
Detection: M4700-07
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No1.B.877
HostMouse
SourceHuman
Concentration~5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHigh molecular weight (>300kD) glycoprotein from human milk-fat globule molecule
SpecificityRecognizes underglycosylated and natural MUC1 protein (isolated from human milk by affinity chromatography). Does not crossreact with normal serum proteins and human tissues of nonepithelial origin. Binds with high efficiency with different epitopes within the VNTR tandem repeat peptide conformations and peptide- carbohydrate compositions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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