Anti -Mucin 1 (Mucin-1, MUC1, MUC-1, Breast Carcinoma-associated Antigen DF3, Carcinoma
Associated Mucin, CD227, DF3, Episialin, Epithelial Membrane Antigen, EMA, H23 Antigen,
H23AG, HGNC:7508, KL-6, MAM6, Peanut-reactive Urinary Mucin, PUM, Polymorphic Epithel
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E IH |
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| MUC-1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in > 90% breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer MUC-1 is overexpressed, is underglycosylated, and the apical localization is lost. MUC-1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kD) and a smaller subunit (14-28kD) noncovalently associated with each other. Transgenic MUC-1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands. MUC-1 can act as a ligand for ICAM-1 on HUVEC cells. It can bind beta-catenin, GSK3beta and it associates with Grb2-SOS upon phosphorylation. Epithelial Membrane Antigen, also referred to as EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin and MUC-1 is a glycoprotein of variable molecular weight, expressed by many glandular epithelial cells. Epithelia of breast, (acini and ducts), exocrine pancreas, bladder, gastrointestinal tracts, thyroid, endometrium and respiratory tract are generally EMA positive. | | | Catalog # | M4700-11Y | | Applications | Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry: Frozen or paraffin sections | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Sp2/0 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | VU-5F12 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in in PBS, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | MUC1 60mer tandem repeat NH2-(VTSAPDTRPAPGSTAPPAHG)3-COOH conjugated to BSA. | | Specificity | Recognizes MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | CA 15-3, CD227 Antigen, Mucin 1 Cell Surface Associated |
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Download Specifications for Anti -Mucin 1 (Mucin-1, MUC1, MUC-1, Breast Carcinoma-associated Antigen DF3, Carcinoma Associated Mucin, CD227, DF3, Episialin, Epithelial Membrane Antigen, EMA, H23 Antigen, H23AG, HGNC:7508, KL-6, MAM6, Peanut-reactive Urinary Mucin, PUM, Polymorphic Epithel |
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