| Secreted epithelial mucins are large macromolecules which exhibit extreme polydispersity. Mucin 2 is the major intestinal mucin. O-glycans are attached to MUC2 in a potentially diverse arrangement, which is crucial for their interaction with endogeneous and exogeneous lectins. Mucins are heavily glycosylated glycoproteins, which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6,7 and 8) have been identified. Various immunohistochemical and in situ hybridization studies have shown that these mucins are differentially expressed in epithelia with cell-type specificity. Muc-5AC and Muc-6 glycoproteins are the first two mucins found in superficial epithelium and Muc-6 glycoprotein is found in the deep glands. |
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| Catalog # | M4700-26 |
| Applications | Suitable for Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilutions | Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 20 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Positive Control | Colon cancer cell line LS174T. Small intestine. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a,k |
| Clone No | 3F279 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.2mg/ml |
| Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M4700-26X. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Recombinant protein encoding 5 tandem repeats of the human MUC2 apomucin. Cellular Localization: Cytoplasmic and cell surface. |
| Specificity | Recognizes human Mucin 2 (MUC2 Glycoprotein). Reacts strongly with colon cancer and gastric cancer. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
