| Secreted epithelial mucins are large macromolecules which exhibit extreme polydispersity. Mucin 2 is the major intestinal mucin. O-glycans are attached to MUC2 in a potentially diverse arrangement, which is crucial for their interaction with endogeneous and exogeneous lectins. |
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| Mucins are heavily glycosylated glycoproteins, which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6, 7 and 8) have been identified. Various immunohistochemical and in situ hybridization studies have shown that these mucins are differentially expressed in epithelia with cell-type specificity. Muc-5AC and Muc-6 glycoproteins are the first two mucins found in superficial epithelium and Muc-6 glycoprotein is found in the deep glands. |
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| Catalog # | M4700-26X |
| Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-2ug/ml for 2hrs at RT. |
| Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Colon cancer cell line LS174T. Small intestine. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a,k |
| Clone No | 3F279 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See M4700-26. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Recombinant protein encoding 5 tandem repeats of the human MUC2 apomucin. MW of Antigen |
| Specificity | Recognizes human Mucin 2. Reacts strongly with colon cancer and gastric cancer. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
