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You are here:Home » Antibodies » Abs to Mucin » Anti -Mucin 2 (MUC2)

Anti -Mucin 2 (MUC2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Catalog #M4700-27A
ApplicationsSuitable for use in Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: Frozen and Paraffin. This product does not require protein digestion pre-treatment of paraffin sections or antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsI1904-65W: IgG, F(abí)2 (HRP) Pab Rb xMo
I1904-66C: IgG, F(abí)2 X-adsorbed (HRP) Pab Rb xMo
Recommended Positive Control Normal colon tissue
HybridomaSpleen cells from immunized Balb/c mice were fused with cells of a mouse NSO myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2743
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenMUC2 tandem repeat peptide.
SpecificityRecognizes the human MUC2 mucin. The antibody recognizes malignant colonic mucosa as well as normal mucosa. In tissue sections, recognizes colon, liver and prostate tissues strongly. Shows no crossreactivity with MUC1, MUC3 or MUC4 mucins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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