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Anti -MUC2 (Mucin-2, MUC-2, Intestinal Mucin-2, SMUC)
Anti -MUC2 (Mucin-2, MUC-2, Intestinal Mucin-2, SMUC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH |
|
| Secreted epithelial mucins are large macromolecules, which exhibit extreme polydispersity. Mucin 2 is the major intestinal mucin. O-glycans attached to MUC2 in a potentially diverse arrangement, which is crucial for their interaction with endogeneous and exogeneous lectins. This antibody reacts strongly with colon cancer and gastric cancer. | | | Catalog # | M4700-28C | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1-2ug/ml for 2h, RT | | Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT | | Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min | | Optimal dilutions to be determined by the researcher. | | Positive Control | Colon cancer cell line LS174T, small intestine | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | SPM296 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant corresponding to 5 tandem repeats of the human MUC2 apomucin. | | Specificity | Recognizes human Muc-2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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