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You are here:Home » Antibodies » Abs to Mucin » Anti -MUC2 (Mucin-2, MUC-2, Intestinal Mucin-2, SMUC)

Anti -MUC2 (Mucin-2, MUC-2, Intestinal Mucin-2, SMUC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Secreted epithelial mucins are large macromolecules, which exhibit extreme polydispersity. Mucin 2 is the major intestinal mucin. O-glycans attached to MUC2 in a potentially diverse arrangement, which is crucial for their interaction with endogeneous and exogeneous lectins. This antibody reacts strongly with colon cancer and gastric cancer.
Catalog #M4700-28C
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2h, RT
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlColon cancer cell line LS174T, small intestine
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone NoSPM296
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant corresponding to 5 tandem repeats of the human MUC2 apomucin.
SpecificityRecognizes human Muc-2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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