M4701-05M Mouse Anti-Mucin 5AC (MUC5AC, Apomucin, Gastric Mucin, Major Airway Glycoprotein, MUC5, Mucin 5 Subtype AC Tracheobronchial, Mucin 5 Subtypes A and C, Mucin 5 Subtypes A and C Tracheobronchial/gastric, Mucin 5AC Oligomeric Mucus/gel Forming, Tracheobronchial Mucin, TBM, Lewis B Blood Group Antigen, LeB)
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry (frozen): Strongly positive on ethanol fixed epithelial cells. Immunohistochemistry (paraffin): Very strong positive reaction with ethanol fixed tissues. On formalin postfixed tissues, a protease pretreatment is recommended. Western Blot: Strongly positive without b mercaptoethanol pretreatment of mucin solution. Optimal dilutions to be determined by the researcher.
Hybridoma
SP2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity
Purified
Specificity
Recognizes peptide core of gastric mucin (MUC5AC).
References
1. Cancer Res.46: 3983-3989 (1986). 2. Biochem. J. 254: 185-193 (1988). 3. Int. J. Cancer 47: 304-310 (1991). 4. J. Immunol. Methods 149: 105-113 (1992) 5. Biochem. J. 305: 211 219 (1995).
TDS
NCBI
UniProt
Tech Support
Customer Service
