| Mucins are heavily glycosylated glycoproteins, which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6,7 and 8) have been identified. Various immunohistochemical and in situ hybridization studies have shown that these mucins are differentially expressed in epithelia with cell-type specificity. Muc-5AC and Muc-6 glycoproteins are the first two mucins found in superficial epithelium and Muc-6 glycoprotein is found in the deep glands. The epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins. |
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| Catalog # | M4701-06 |
| Applications | Suitable for use in Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 min at RT. No special pretreatment is required for |
| immunohistochemical staining of formalin-fixed tissues. |
| Optimal dilutions to be determined by the researcher. |
| Epitope | The epitope is destroyed by b-mercaptoethanol and proteases treatment. It is not destroyed by periodate treatment. |
| Positive Control | Stomach |
| Cellular Localization | Cytoplasmic and cell surface |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 0.N.458 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.2mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Available without BSA and azide. See M4701-06X. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b-) patient. Cellular Localization: Cytoplasmic and cell surface |
| Specificity | Recognizes the peptide core of gastric mucin M1 (> 1000kD) (recently identified as Mucin 5AC). Species Crossreactivity: Human, monkey, rabbit, feline, mouse, rat, chicken and bovine. Does not crossreact with porcine or hedgehog. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
