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Anti -MUCDHL (Mucin and Cadherin-Like Protein, mu-Protocadherin)
Anti -MUCDHL (Mucin and Cadherin-Like Protein, mu-Protocadherin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| Mucin and cadherin-like protein (MUCDHL), also known as -protocadherin, is a 110kD type I transmembrane protein that is both O- and N-glycosylated. It is a calcium-dependent cell adhesion protein in the cadherin superfamily. MUCDHL is found on intestinal and renal epithelium. Human MUCDHL extracellular domain (ECD) contains four cadherin domains (residues 71-459) plus several mucin domains (residues 540-645). Alternate splicing creates four isoforms that differ in their mucin domains. In the region used as immunogen, human and mouse MUCDHL share 63% amino acid sequence identity. | | | Catalog # | M4703-10 | | Applications | Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human MUCDHL. The detection limit for rhMUCDHL is approximately 5 ng/lane under non-reducing and reducing conditions. | | Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human MUCDHL. The detection limit for rhMUCDHL is approximately 0.3 ng/well. | | Optimal dilutions should be determined by each laboratory for each application. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml. | | Purity | Purified by human MUCDHL affinity chromatography. | | Immunogen | E. coli-derived rhMUCDHL (aa26-434) | | Specificity | Selected for its ability to recognize human MUCDHL in direct ELISAs and Western Blots. In these formats, this antibody shows ~10% crossreactivity with rmMUCDHL. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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