Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Cadherin » Anti -MUCDHL (Mucin and Cadherin-Like Protein, mu-Protocadherin)

Anti -MUCDHL (Mucin and Cadherin-Like Protein, mu-Protocadherin)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B FC
Mucin and cadherin-like protein (MUCDHL), also known as mu-protocadherin, is a 100kD type I transmembrane protein belonging to the protocadherin family of cell adhesion molecules. It is found apically on the brush border of renal proximal tubule epithelial cells. The extracellular domain of mature mouse MUCDHL contains four cadherin domains plus three repeat regions that constitute a mucin stalk (aa 541-614). At least one splice variant exists in mouse that shows a deletion of aa's 459-620. Over aa's 29-479, mouse MUCDHL shares 63% and 79% aa identity with human and rat MUCDHL, respectively.
Catalog #M4703-11
ApplicationsSuitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications not tested.
Recommended DilutionWestern Blot: Can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for rmMUCDHL is approximately 1 ng/lane under non-reducing and reducing conditions.
Flow Cytometry: Tested in flow cytometry using primary kidney cells. Dilute this antibody to 25ug/ml and add 10 L of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome.
Direct ELISA: Can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for rmMUCDHL is approximately 0.5 ng/well.
Optimal dilutions to be determined by the researcher.
ReconstitutionReconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS with 5% trehalose.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, NS0-derived rmMUCDHL (rmMUCDHL; aa 29 - 479; Accession # NP_082345)
SpecificityRecognizes mouse MUCDHL. In direct ELISAs and Western Blots, this antibody shows approximately 5% crossreactivity with rhMUCDHL.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links