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You are here:Home » Antibodies » Abs to RNA Processing » Anti -Muscleblind-like 1 (MBNL1, EXP35, EXP40, EXP42, Muscleblind-like, MBNL, MBNL Protein, Muscleblind 41kD Isoform, Triplet Expansion RNA Binding Protein, DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428)

Anti -Muscleblind-like 1 (MBNL1, EXP35, EXP40, EXP42, Muscleblind-like, MBNL, MBNL
Protein, Muscleblind 41kD Isoform, Triplet Expansion RNA Binding Protein, DKFZp686P06174,
EXP, EXP35, EXP40, EXP42, KIAA0428)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Several important human diseases are associated with expansion of polynucleotide sequences, in most cases trinucleotide repeats. Myotonic dystrophy (DM1) is one of these diseases, and is associated with increases in the number of CTG repeats in the 3' UTR of the gene encoding myotonin (a.k.a. DM Kinase, DMK or myotonin-Protein Kinase), a ser/thr kinase expressed specifically in muscle. Mammalian genomes contain three muscleblind-like proteins, generally referred to as MBNL1, MBNL2 and MBNL3, and all three were found to associate with long double stranded CUG RNA repeats, thus, being triplet repeat expansion dsRNA-binding proteins.
Catalog #M9426-01B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionPeptide ELISA: 1:,000.
Western Blot: 1-3ug/ml. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See M9426-01B-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenA synthetic peptide from the C-terminus of aa-SAEHLTSHKYVTQ
SpecificityRecognizes human MBNL1. Species sequence homology: mouse, rat, canine, bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesMBNL1; muscleblind-like (Drosophila); DKFZp686P06174; EXP; EXP35; EXP40; EXP42; KIAA0428; MBNL; muscleblind-like 1


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